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It makes use of the fact that every living cell (e.g., animal, plant, bacteria, yeast, and mold) contains ATP for energy. It is based on the firefly’s ATP luminescent reaction. Firefly has two chemical compounds, Luciferin and Luciferase, that react with the insect’s ATP to produce bioluminescence light.
ATP collected from a contact surface reacts with Luciferin & Luciferase compounds present in the swab sample to create bioluminescence light. The amount of bioluminescence light is measured by the Luminometer and is expressed in RLUs (Relative Light Units).
Yes, the higher RLU indicates improper cleaning and the presence of contamination in the form of high microbes or high food residue. It also indicates the need for immediate corrective actions.
ATP testing should be done after the cleaning, but before sanitization.
Advantages:
Limitations:
None
The article is quite insightful. One question: why is it important to perform the ATP swab test before sanitization?
It is important to test and customize RLU limits for your testing program. Any score of 10 RLU or less is a Pass. Any score greater than 10 RLU is a Fail. The greater the ATP, the higher the RLU. These default limits are based on years of experience in the food and beverage industry. Thank you for your question!
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